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Activity Test
OBJECTIVE
Verify the antifreeze activity of the purified LpAFP protein in the presence of a nucleating agent at different temperatures and concentrations, and compare it with other substances.
WHAT IS DONE TO ACHIEVE IT?
(1) A nucleating agent is placed in a glass tube.
(2) 5 mL of water or solutions under study are added to the tubes.
(3) The tubes are immersed in a recirculating water/ethanol bath at a certain temperature between 0 and -10 °C. After 30 minutes, the tubes are shaken vigorously and observe which tubes form ice.
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Water
NaCl 3 Osmol/L
Glycerol 3 Osmol/L
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